The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR items were digested and cloned right into a pET21a+ vector making use of NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the https://17-hydroxy-sprengerinin-c45554.blogdigy.com/agx51-for-dummies-40334400